 
Professor
of Microbiology
BS 1970,
University of Minnesota
DVM 1972, University of Minnesota
PhD 1976, University of Georgia
Diplomate, American College of Veterinary Microbiologists
 
Senior Scientist
BA 1977,
Stanford University
MBA, MPH 1981, Yale University
DVM 1994, University of Wisconsin
 Johne's
disease is a prevalent, infectious and usually fatal disease primarily
affecting ruminants (cattle, sheep, goats, deer, bison, etc.). Improving
methods of detecting the infection is the primary focus of the Johne's
Testing Center (JTC). The JTC processes thousands of samples submitted
from throughout the USA and the diagnostic techniques available
in our laboratory are used to study the epidemiology, economic impact,
zoonotic potential, and pathogenesis of the disease in a variety
of animal species. In addition to its own research program, the
JTC collaborates with human and veterinary medical scientists from
many countries and types of institutions. The JTC provides information
and education on Johne's disease through telephone consultation,
herd visits, veterinary and producer presentations, lay publications,
and this web site.
Representative
publications from our laboratory
- Corn, J.L., M.E. Cartwright, K.J Alexy, T.E. Cornish, E.J.B. Manning, A.N. Cartoceti, & J.R. Fisher. 2010. Surveys for Disease Agents in Introduced Elk in Arkansas and Kentucky. Journal of Wildlife Diseases, 46(1), 186-194.
- Naser, S.A., M.T. Collins, J.T. Crawford, and J.F. Valentine. 2009. Culture of Mycobacterium avium subspecies paratuberculosis *MAP) from the blood of patients with Crohn’s disease: a follow-up blind multicenter investigation. The Open Inflammation Journal 2:22-23.
- Krishnan, Y.K., E.J.B. Manning, and M.T. Collins. 2009. Comparison of three methods for susceptibility testing of Mycobacterium avium subsp. paratuberculosis to 11 antimicrobial drugs. J. Antimicrobial. Chemotherapy. 64(2):310-316.
- Pedersen K, Manning E.J.B. and Corn, L.J. Distribution of Mycobacterium avium subspecies paratuberculosis in the lower Florida keys. J. Wildl. Dis. 2008 44: 578-584.
- Sleeman J.M., Manning E.J.B., Rohm J.H., Sims J.P., Sanchez S. Gerhold R.W. and Keel M.K. Johne's disease in a free-ranging white-tailed deer from Virginia and subsequent surveillance for Mycobacterium avium subspecies paratuberculosis. J. Wildl. Dis. January 2009.
- Shin, S.J. and M.T. Collins. 2008. Thiopurine drugs azathioprine and 6-mercaptopurine inhibit Mycobacterium paratuberculosis growth in vitro. Antimicrob. Agents Chemother. 52:418-426.
- Osterstock, J.B.; G.T. Fosgate, N.D. Cohen, J.N. Derr, E.J.B. Manning, M.T. Collins, and A.J. Roussel. 2008. Familial associations with paratuberculosis ELISA results in Texas Longhorn cattle. Vet. Micro. 129(1-2), 131-138.
- Collins. M.T. 2007. Johne’s Disease: Practical Solutions and Problem Solving (part 1) and Successful Control of Johne’s Disease in Nine Dairy Herds: Results of an ongoing field trial (part 2). pp 61-68, Proceedings of the 116th annual meeting of the South Dakota Veterinary Medical Association. Sioux Falls, SD August 12-15, 2007.
- Shin SJ, Han JH, Manning EJB, Collins MT. 2007. Rapid and reliable method for quantification of M. avium ss. paratuberculosis by use of the BACTEC MGIT 960 system. J. Clin. Micro. 45(6): 1941-1945.
- Wu CW, Livesey M, Schmoller SK, Manning EJ, Steinberg H, Davis WC, Hamilton MJ, Talaat AM. 2007. Invasion and persistence of Mycobacterium avium subsp. paratuberculosis during early stages of Johne's disease in calves. Infect Immun. May 75(5):2110-9
- Manning, E.J.B., H. Cushing, S. Hietala, C. Wolf. 2007. Impact of Corynebacterium pseudotuberculosis infection on serologic surveillance for Johne’s disease in goats. J. Vet. Diagn. Invest. 19: 187-190.
- Crawford, GC, Ziccardi MH, Gonzales BJ, Woods LM, Fischer JK, Manning EJB, Mazet J. 2006. Mycobacterium avium ssp. paratuberculosis and Mycobacterium avium ssp. avium infections in a tule elk (Cervus elaphus nannodes) herd. J. Wildlife Diseases 42(4) 715-723.
- Salgado, M., J. Kruze, and M.T. Collins. 2007. Diagnosis of paratuberculosis by fecal culture and ELISA on milk and serum samples in two types of Chilean dairy goat herds. J. Vet. Diagn. Invest. 19:99-102.
- Michael T. Collins, Ian A. Gardner, Franklyn B. Garry, Allen J. Roussel, Scott J. Wells. Consensus recommendations on diagnostic testing for the detection of paratuberculosis in cattle in the United States Journal of the American Veterinary Medical Association, December 15, 2006, Vol. 229, No. 12, Pages 1912-1919.
- Cho, D. and M. T. Collins. 2006. Comparison
of the Proteosomes and Antigenicities of Secreted and Cellular
Proteins Produced by Mycobacterium paratuberculosis. Clin.Vaccine
Immunol. 13:1155-1161.
- Wells, S. J., M. T. Collins, K. S. Faaberg, C. Wees,
S. Tavornpanich, K. R. Petrini, J. E. Collins, N. Cernicchiaro,
and R. H. Whitlock. 2006. Evaluation
of a Rapid Fecal PCR Test for Detection of Mycobacterium
avium subsp. paratuberculosis in Dairy Cattle. Clin.Vaccine Immunol. 13:1125-1130.
-
Kruze, J., M. Salgado, E. Paredes,
A. Mella, M.T. Collins. 2006. Goat paratuberculosis in Chile:
first isolation and confirmation of Mycobacterium avium subspecies paratuberculosis infection
in a diary goat herd. J. Vet. Diagn. Invest. 18:476-479.
- Dorshorst N.C., M.T. Collins, and
J.E. Lombard. 2006. Decision
analysis model for paratuberculosis control in commercial
dairy herds. Prev. Vet. Med. 75:92-122.
- Wu,
C-W., J. Glasner, M. T. Collins, S. Naser, and A. M. Talaat.
2006. Whole-genome
plasticity among Mycobacterium
avium subspecies: Insights from comparative genomic
hybridizations. J. Bacteriol. 188:711-723.
- Gonda,
M.G., Y.M. Chang, G.E. Shook, M.T. Collins, and B.W. Kirkpatrick.
2006. Genetic
variation of Mycobacterium
avium ssp. paratuberculosis infection
in US Holsteins. J. Dairy Sci. 89:1804-1812.
- Corn,
J.L., E.J.B. Manning, S. Sreevatsan, and J.R. Fischer.
2005.
Isolation
of Mycobacterium
avium subsp. paratuberculosis from
free-ranging birds and mammals on livestock premises. Appl.
Environ. Microbiol. 71(11):6963-6967.
- Salgado, M., E.J.B. Manning, and
M.T. Collins. 2005. Performance
of a Johne’s disease
enzyme-linked immunosorbent assay adapted for milk samples
from goats. J. Vet. Diagn. Invest. 17:350-354.
- Collins, M.T., S.J. Wells, K.R.
Petrini, J.E. Collins, R.D. Schultz, and R.H. Whitlock. 2005. Evaluation
of five antibody detection tests for diagnosis of bovine
paratuberculosis. Clin. Diagn. Lab. Immunol. 12: 685-692.
- Ghadiali,
A. H., M. Strother, S. A. Naser, E. J. B. Manning, and
S. Sreevatsan. 2004. Mycobacterium
avium subsp. paratuberculosis strains
isolated from Crohn's disease patients and animal species
exhibit similar polymorphic locus patterns. J. Clin.
Microbiol. 42:5345-5348.
- Davidson,
W. R., E. Manning, and V. Nettles. 2004. Culture
and Serologic Survey for Mycobaterium avium subsp. paratuberculosis
Infection among Southeastern White-tailed Deer. Journal
of Wildlife Diseases. 40(2): 301-306
- Motiwala,
A. S., A. Amonsin, M. Strother, E. J. B. Manning, V. Kapur, and
S. Sreevatsan. 2004. Molecular
Epidemiology of Mycobacterium avium subsp. paratuberculosis
Isolates Recovered from Wild Animal Species. J.Clin.Microbiol.
42:1703-1712.
- Sung,N.,
K. Takayama, and M.T. Collins. 2004. Possible
association of GroES and antigen 85 proteins with heat resistance
of Mycobacterium paratuberculosis. Appl. Environ.
Microbiol. 70:1688-1697.
- Carpenter,
T.E., I.A. Gardner, M.T. Collins, and R.H. Whitlock. 2004. Effects
of prevalence and testing by enzyme-linked immunosorbent assay
and fecal culture on the risk of introduction of Mycobacterium
avium subsp. paratuberculosis-infected cows into
dairy herds. J. Vet. Diagn. Invest. 16:31-38.
- Collins,
M.T. 2004. Update
on paratuberculosis: 3. Control and zoonotic potential. Irish
Veterinary Journal 57(1): 49-52.
- Collins,
M.T. 2003. Update
on paratuberculosis: 2. Pathology and diagnosis. Irish Veterinary
Journal 56(12): 619-623.
- Collins,
M.T. 2003. Update
on paratuberculosis: 1. Epidemiology of Johne's disease and
the biology of Mycobacterium paratuberculosis.
Irish Veterinary Journal 56(11):565-574.
- Collins,
M.T. 2003. Paratuberculosis:
Review of present knowledge. Acta Vet. Scand. 44:217-221
- Sung,
N., and M.T. Collins. 2003. Variation
in resistance of Mycobacterium paratuberculosis to
acid environments as a function of culture medium. Appl.
Env. Microbiol. 69:6833-3840.
- Manning,
E.J.B., H. Steinberg, V. Krebs, M.T. Collins. 2003. Diagnostic
testing patterns of natural Mycobacterium paratuberculosis
infection in pygmy goats. Can. J. Vet. Res. 67:213-218.
- Manning,
E.J.B., T.E. Kucera, N.B. Gates, L.M. Woods, and M. Fallon-McKnight.
2003. Testing
for Mycobacterium avium subsp. paratuberculosis
infection in asymptomatic free-ranging tule elk from an infected
herd. J. Wildlife Diseases 39:323-328.
- Collins,
M.T. 2003. Summation.
pp 531-535 in Proceedings of the 7th International Colloquium
on Paratuberculosis. Bilbao, Spain June 12-14, 2002. R.A. Juste,
M.V. Geijo, and J.M. Garrido, editors. International Association
for Paratuberculosis, Madison, WI USA.
- Kramsky,
J.A., E.J.B. Manning, and M.T. Collins. 2003. Protein
G binding to enriched serum immunoglobulin from nondomestic
hoofstock species. J. Vet. Diagn. Invest. 15:253-261.
- Manning,
E.J.B., M. Augenstein, M.T. Collins, and K.M. Nelson. 2003. Case
report - Johne’s disease: the recipient risk. The Bovine
Practitioner 37: 20-22.
- Quist,
C.F., V.F Nettles, E.J.B. Manning, D.G. Hall, J.K. Gaydos, T.J.
Wilmers, R.R. Lopez. 2002. Paratuberculosis
in key deer (Odocoileus virginianus clavium).
J. Wildlife Dis. 38:729-737.
-
- Zwick,
L.S., T.F. Walsh, R. Barbiers, M.T. Collins, M.J. Kinsel, and
R.D. Murnane. 2002. Paratuberculosis
in a mandrill (Papio sphinx). J. Vet. Diagn. Invest.
14:326-328.
-
- Kalis,
C.H.J., J.W. Hesselink, H.W. Barkema, M.T. Collins. 2001. Use
of long-term vaccination with a killed vaccine to prevent fecal
shedding of Mycobacterium avium subsp paratuberculosis.
Am. J. Vet. Res. 62:270-274.
-
-
-
Kramsky,
J.A.*, D.S. Miller, A. Hope, M.T. Collins. 2000.
Modification of a bovine ELISA to detect camelid antibodies
to Mycobacterium paratuberculosis. Vet. Microbiol. 77:333-337.
-
-
Collins, M. T., G. Lisby,
C. Moser, D. Chicks, S. Christensen, M. Reichelderfer,
N. Høiby, B.A. Harms, Ø.O. Thomsen, U.
Skibsted, and V. Binder. , 2000. Results
of multiple diagnostic tests for Mycobacterium avium subsp. paratuberculosis in
patients with inflammatory bowel disease and controls.
J. Clin. Microbiol. 38:4373-4381.
-
Buergelt, C.D., A.W. Layton,
P.E. Ginn, M. Taylor, J.M. King, P.L. Haebecker, E. Mauldin,
R. Whitlock, C. Rossiter, and M.T. Collins. 2000. The
pathology of spontaneous paratuberculosis in North American
Bison (Bison bison). Vet. Pathol. 37:428-438.
- Collins,
M.T. 1999. Spreadsheet
model for estimating the probability herds are free of paratuberculosis
after successive serial tests. pp 66-75 in Proceedings of
the 6th International Colloquium on Paratuberculosis. Melbourne,
Australia February 14-18, 1999. E.J.B. Manning and M.T. Collins,
editors. International Association for Paratuberculosis, Madison,
WI USA.
-
Zhao,
B.Y., C.J. Czuprynski, and M.T. Collins. 1999. Intracellular
fate of Mycobacterium avium subspecies paratuberculosis
in monocytes from normal and infected, interferon-responsive
cows as determined by a radiometric method. Can.J.Vet.Res.
63:56-61.
- Manning,
Elizabeth and Collins, MT. 1999. Paratuberculosis in zoo animals.
In: Fowler, ME and ER Miller (eds). Zoo and Wild Animal Medicine:
Current Therapy 4, W.B. Sauders Company, Philadelphia,
612-616.
-
- Manning,
EJB, Steinberg H, Rossow K, Ruth, GR Collins MT. 1998. Epizootic
of a paratuberculosis outbreak in farmed elk (Cervus elaphus).
J Am Vet Med Assoc. 213, 1320-1322.
- Collins,
M.T., and E.J.B. Manning. 1998. Results
of serological testing of AABP members for antibody to M. paratuberculosis.
Proceedings of the 31st Annual Conference of the American Association
of Bovine Practitioners, September 24-26, Spokane Washington.
-
- Collins,
M.T. 1994. Clinical
approach to control of bovine paratuberculosis. J Am
Vet Med Assoc. 204: 208-210.
- Collins,
M.T., Sockett, D.C., Goodger, W.J., et al. 1994. Herd
prevalence, geographic distribution of, and risk factors
for, bovine paratuberculosis in Wisconsin. J Am Vet Med
Assoc. 204:636-641.
-
Collins,
M.T. and Sockett, D.C.. 1993. Accuracy and economics
of the USDA-licensed enzyme-linked immunosorbent assay
for bovine paratuberculosis. J. Am Vet Med Assoc. 203:1456-1463.
- Lambrect, R.
S. and M. T. Collins. 1993. Inability
to detect mycobactin in Mycobacteria-infected tissues suggests
an alternative iron acquisition mechanisms by Mycobacteria in
vivo. Microb. Pathog. 14:229-238.
-
- Collins,
M.T., Morgan, I.R. 1992. Simulation
model of paratuberculosis control in a dairy herd. Prev Vet
Med 14:21-32.
- Collins, M. T. and I. R. Morgan.
1991. Epidemiologic
model of paratuberculosis in dairy cattle. Prev. Vet. Med.
11:131-146.
- Collins, M. T. and I. R. Morgan.
1991. Economic
decision analysis model of a paratuberculosis test and
cull program. J. Am.
Vet. Med. Assoc. 199:1724-1729.
- Sockett,
D. C., D. J. Carr, and M. T. Collins. 1992. Evaluation of conventional
and radiometric fecal culture and a commercial DNA probe for
diagnosis of Mycobacterium paratuberculosis infections
in cattle. Can.J.Vet.Res. 56:148-153.
- Van
Boxtel, R.M., R.S. Lambrecht, and M.T. Collins. 1990. Effect
of polyoxyethylene sorbate compounds (Tweens) on colonial
morphology, growth, and ultrastructure of Mycobacterium
paratuberculosis. APMIS 98:901-908.
- Damato,
J. J. and M. T. Collins. 1990. Growth
of Mycobacterium paratuberculosis in radiometric,
Middlebrook and egg-based media. Vet.Microbiol. 22:31-42.
- Collins,
M. T., K. B. Kenefick, D. C. Sockett, R. S. Lambrecht, J. McDonald,
and J. B. Jrrgensen. 1990. Enhanced
radiometric detection of Mycobacterium paratuberculosis
using filter concentrated fecal specimens. J.Clin.Microbiol.
28:2514-2519.
- Lambrecht,
R. S., J. Carriere, and M. T. Collins. 1988. A
model for analyzing growth kinetics of a slowly growing Mycobacterium
sp. Appl.Env.Microbiol. 54:910-916.
|
