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Colonies
of M. paratuberculosis
on culture medium without Tween
Colonies
of M. paratuberculosis grown on Middlebrook agar media without
Tween are rough in appearance. Tween 80 is a surfactant commonly
used in culture media to accelerate the growth of mycobacteria.
For M. paratuberculosis, Tween 80 can alter the colonial
and cellular morphology.
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