TESTING SERVICES - JOHNE'S INFORMATION CENTER

SUBMISSIONS | TESTS

Greetings from the nationally approved
JOHNE’S TESTING CENTER!

We’re here at the School of Veterinary Medicine, University of Wisconsin* to help keep your herds healthy and productive. After two decades of service, we continue to lead the industry in diagnostic testing, responsiveness to clients and Johne’s disease education. We provide an array of services to make your life easier and to keep your costs low:

Would you like to use the most accurate testing methods? We do them all!

Blood tests (ELISA, AGID)
Individual and pooled fecal culture (we’re the only lab in the state that offers this assay as a regular service!)
Culture of tissue and environmental samples as soil or water
Multiplex PCR
Milk ELISA

How would you like to submit blood samples? Any tube size is fine with us!
How would you like to receive your confidential test results: standard mail? Fax? Email? By the web? Just let us know your preference!
What about volume discounts? We’ve got them!
Want to talk with an expert about the test results, about your state’s voluntary Johne’s disease control program or how to manage the infection in your herd? There is always someone available to discuss any aspect of this challenging infection with you (confidentially of course). Give us a call, send us an email, or feel free to drop by.

We’ll help your herd be Johne’s free!

Services provided by the JTC beyond testing include:

Education: We help you understand the biology and spread of this infection in whatever way works best for you: meeting in person or with your interest group, discussing the topics most relevant to you on the phone, responding promptly to questions sent in by email or through "Ask An Expert” on this website.

Testing strategies: We can tailor testing strategies that make sense specifically for your herd and budget. For instance, pooling of fecal samples can cut the cost of a whole herd test to 1/5 of individual animal testing. In a milking herd of 100, that’s a whole herd test at $500 vs. $2,500.

Find resources: We can identify resources to support your efforts to keep Johne’s under control (state programs, risk assessments, equipment grants, etc.)

Veterinarian support: We can provide you with results by animal ID, by result, by animal age or by any other herd element that is useful to you in spreadsheet format. We’d be happy to analyze the results on a herd basis to help you design the best management approach for each of your clients.

We look forward to providing you the same excellent service enjoyed by our long term clients. Please call 608-263-6920 with any questions you may have about these services.

Use this form to submit your samples:

Culture & Serology
for Diagnosis

Send your samples to:

Johne's Testing Center
School of Veterinary Medicine*
2015 Linden Drive
Madison, WI 53706-1102

*(not Easterday Drive ; Next door to, but distinct from, WVDL)

Questions concerning Johne's disease and testing can be directed Monday through Friday by phone or fax to 608-26-JOHNE. You may also submit a question through "Ask An Expert”

Each submission (no matter how many samples per submission) is charged a single accession fee of $5.00. This fee covers sample handling, paperwork, provision of results, data management, etc.

SERUM and MILK ANTIBODY ASSAYS (ELISA, AGID)
These assays test for antibody produced by the animal in response to infection with M. paratuberculosis. Not for use with serum or milk from Johne’s disease vaccinated animals.

Costs:

Serum and Milk ELISAs -

1 - 10 $8.00 each

11 - 99 $6.00 each

> 100 $5.00 each

AGID - $10.00 each

Sample:

1-2 ml serum.
Please send serum in a separator tube after centrifugation or decanted into a leakproof container. Collect milk aseptically – you can just pour off an aliquot from your DHI sample.

Shipping:

Overnight/second day UPS/FedEx on cold packs as soon after collection as possible, to arrive Mon–Fri before 4:00 pm.

Results:

Results will be available within 3 days of sample receipt.

ISOLATION OF M. PARATUBERCULOSIS
(from any type of sample: fecal, tissue, water, soil, milk, etc)

Cost:

Individual fecal, tissue, environmental sample

$30 each, includes PCR confirmation of M. paratuberculosis

Pooled fecal samples (send individual samples; JTC lab personnel will create the pools)

Five samples per pool
Includes PCR confirmation of M. paratuberculosis
Follow-up with individual sample re-testing (stored at JTC) if requested; storage fee $3.00/sample. Call the lab before shipment to request this service.

1 - 10 pools		$30 each pool
11 – 99 pools		$25 each pool
> 100 pools		$20 each pool

Sample:

3 grams of feces (approximately 1 tablespoon) or 3 grams of tissue (at least 1 square inch) from any animal species in sterile, sealable plastic bags or other wide-mouthed, leak-proof containers. The container should be labeled with the animal ID. For culture of samples other than fecals or tissue, call before collection.

Shipping:

Overnight UPS/Fed Ex on cold packs as soon after collection as possible, to arrive Mon–Fri before 4:00 pm. Samples may be refrigerated overnight.

Results:

Once isolated from culture, mycobacterial (acid-fast) organisms will be confirmed as M. paratuberculosis by multiplex PCR. Isolation results for this slowly growing organism will be available within 1 to 8 weeks.

DIRECT FECAL SAMPLE PCR

$50 per sample. Submit fecal sample as directed above for fecal culture.